Cytovale

Technology

Three decades of work in labs spanning the globe tell us that the biomechanical properties of cells are intimately linked with their underlying state

Biomechanical properties of single cells

(e.g., cellular deformability, or cellular viscosity)

Indicate changes in the global state of cells, including phenomena such as:

Proliferation

Highly replicative cancer cells are significantly more deformable than their non-cancerous, benign, precursors.

Activation

Immune activation can be readily distinguished from baseline biomechanical signatures, and chronic vs. acute inflammation states have notable differences.

Differentiation

Pluripotent stem cells become less deformable as they commit to a lineage and differentiate.

Migration

Metastatic cancer cells are notably more deformable than those with reduced metastatic potential.

Comparison of Leading Techniques to Assess Cell Mechanics

The main issue to date has been throughput

Generating meaningful diagnostic information requires the analysis of thousands of cells. Until deformability cytometry, most techniques could only measure tens of cells per hour, rendering the approach too cumbersome for clinical diagnostic applications or high throughput research applications.

Technique
Cost
Throughput

Atomic Force Microscopy

Complex Operation

$$$

1 cell / 15 min

Magnetic Twisting Cytometry

Multi-Step

$$

1 cell / sec

Optical Stretching

Low Reproducibility

$$

1 cell / min

Cell Transit Analyzers

Prone to Clogging

$

1 cell / sec

CytoVale Mechanomics Platform

Robust, Single-Step

¢

2000 cells / sec

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Cytovale System in Diagnostics

From Patient to Answer in Under 10 Minutes

Diagnostic

Patient

Diagnostic uncertainty means optimal treatment course is unknown.

Diagnostic

Fluid Sample

A fluid sample containing the diagnostic cells of interest is collected from the patient.

Diagnostic

Cartridge Preparation

The sample is loaded on a single-use, microfluidic cartridge with proprietary features for assessing the biomechanical properties of single cells.

Diagnostic

Processing

The cartridge is inserted into a benchtop instrument containing a “deconstructed microscope” that actuates precision flow and captures high speed videography data at over 500,000 image frames per second.

Diagnostic

Cellular Squeeze

Under flow, precision microfluidic features apply carefully calibrated forces to the cells, and the resulting deformation is captured with high speed videography.

Diagnostic

Metrics and Analytics

Image analysis captures dozens of metrics for each cell, including morphology metrics prior to squeezing (e.g., cell diameter or surface roughness), molecular metrics from fluorescent cell surface probes (e.g., CD45+), and structural metrics (e.g., maximum cellular deformability) during the squeezing event.

Diagnostic

Disease Signature

Robust disease signatures are constructed across multiple dimensions and are reduced by machine learning techniques. Each patient's unique signature is compared to established profiles to determine a diagnostic score.

Diagnostic

Diagnostic Result

The diagnostic score is reported to the clinician and provides a better lens into the disease state of the patient, enabling appropriate and timely care and reducing morbidity, mortality, and cost.

Want to Learn More?

Read About Our First Clinical Application